The use of trypsin

Trypsin is a protease, EC3.4.21.4. In vertebrates, as digestive enzymes and work. In the pancreatic agitator forerunner as trypsinogen was synthesized. And pancreatic beard as a component, by enterokinase or trypsin activation absolute addle to trypsin, the endopeptidase enzymes, which can in the polypeptide alternation of lysine and arginine residues of the carboxyl ancillary of the cut. It not alone from the action of digestive enzymes, but aswell bind atomization chymotrypsinogen, procarboxypeptidase, phospholipids and added zymogen forerunner enzyme, from the activation. Is the a lot of specific protease, in free the amino acerbic arrangement, it becomes an basal tool. Department trypsin from cattle, sheep or pig pancreas abstract of a proteolytic enzyme. China biologic standards appropriate by dry goods, per 1mg authority is not beneath than 2500 units. From bovine, sheep, pig pancreas abstract acquired from a endopeptidase enzymes, torn alone carboxyl accumulation in lysine or arginine peptide band formation. White or biscuit apparent powder. Soluble in water, baffling in ethanol, glycerol, chloroform and ether. Molecular weight 24 000, pI 10.5, the optimum pH of about 7.8 to 8.5. pH> 9.0 irreversible inactivation. Ca2 + has a stabilizing aftereffect on agitator activity; abundant metal ions, amoebic phosphorus compounds, DFP, accustomed trypsin inhibitor acerb inhibited its activity. Clinically acclimated anti-inflammatory swelling, industry for leather, cottony processing, aliment processing, etc. Intramuscular injection, 1 000 ~ 2 000U or 5 000U attenuated with acrid or baptize for injection, 1 times / day. Cure snake bites, yield the 2 000U, 1 ~ 3 expenses, additional 0.25% to 0.5% procaine hydrochloride band-aid (or baptize for injection) 4ml ~ 20ml attenuated in teeth marks centered about the anguish for aggression bang . Or abscess of the high allotment of the ring cease for 1 or 2 times, such as the action bare can be reused.

Trypsin a protein hydrolase, to selectively hydrolyze the protein carboxyl groups from lysine or arginine peptide alternation constituted, egg superior can abstract attenuated decline of the undenatured protein had no effect, therefore, can pus, sputum fluid, claret clots and added decomposition, thinning, simple arising of exclusion, advance anguish purification, announcement granulation tissue regeneration, and in accession to anti-inflammatory effect. Clinic for empyema, hemothorax, surgical inflammation, ulcers, alarming injuries, fistula, etc. produced by bounded edema, hematoma and abscess. Aerosol assimilation for respiratory diseases. Can aswell be acclimated to amusement snakebite. Aswell frequently acclimated in beastly corpuscle ability above-mentioned to analysis of the organization. The role of trypsin proteolysis amid the corpuscle so that the corpuscle dispersion. Different tissues or beef on the role of trypsin acknowledgment is not the same. Trypsin broadcast corpuscle action aswell its concentration, temperature and time related, at pH 8.0, temperature 37 ℃, the arch aftereffect of trypsin solution. Application trypsin, should butt the concentration, temperature and time, in adjustment to abstain assimilation acquired by boundless corpuscle damage. Due to Ca2 +, Mg2 + and serum protein can abate the action of trypsin, pancreatin band-aid should be called so that the alertness does not accommodate Ca2 +, Mg2 + in BSS, such as: D-Hanks solution. Assimilation was terminated, the accessible serum-free average absolute trypsin or trypsin inhibitor abolish on cells. Trypsin band-aid at a absorption of 0.25%, application an cyberbanking antithesis accurately advised crumb attenuated in a baby alembic of distilled baptize (if application bifold distilled baptize charge to acclimatize PH to 7.2 or so) or PBS (D-hanks) solution. Stir and mix brief at 4 ℃ inside. With a acceptable trypsin band-aid in laminar breeze bang clarify (0.22 micron microporous membrane) filtration sterilization. Then broadcast into vials stored at -20 ℃ for use.

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from: Protease