Dubos (R.J.Dubos, 1939) was added to the soil at 37 ℃ for staphylococcal seen when added staphylococci were dissolved. He was isolated from a soil fungus spores and culture fluid from its precipitation in pH3-4.6 will inhibit the growth of Gram-positive bacteria isolated as a crystal substance named TYROTHRICINE, taken together with the study of penicillin the first step in the study antimicrobial substances. Was divided into tyrothricin from acetone - ether mixture extracted gramicidin (gramicidin) and their residues in casein peptides retained Brevibacterium (tyrocidine).
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