Wortmannin related research

PI3K/Akt inhibitor wortmannin research on leukemia cell proliferation and apoptosis , and to explore its possible mechanism . Methods: The effect of different concentrations of wortmannin in human myeloid leukemia cell K562, using the MTT cell proliferation , single cell gel electrophoresis to detect DNA damage formation of the " comet "-like smearing , AnnexinV-FITC / PI double staining to detect apoptosis , Westernblotting, RT-PCR detection of K562 cells after wortmannin total Akt and phosphorylated Akt and NF-κB gene and protein expression level changes . Results : wortmannin in a time - inhibited dose-dependent manner the proliferation of K562 cells , the IC50 value of 24h 25nmol / L. Wortmannin -induced apoptosis of K562 cells , and its role in a dose -dependent enhancement . Wortmannin in K562 cell DNA strand breaks , presenting " comet " tail phenomenon, its tail length and tail were significantly higher (P0.01). Wortmannin can be both protein and gene level in a dose- dependent manner and inhibited phosphorylation of NF-κB expression of Akt , total Akt protein but not significantly affected. Conclusion: wortmannin time- and dose- dependent manner inhibited the proliferation of K562 cells and induce apoptosis , which may relate to reduced phosphorylation of PI3K/Akt signaling pathway and NF-κB protein expression.


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